Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Pseudomonas sp. | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | requirement | Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
1 * 39000 + 1 * 37000, SDS-PAGE, amino acid analysis, N-terminal amino acid sequence | Pseudomonas sp. |
39000 | - |
1 * 39000 + 1 * 37000, SDS-PAGE, amino acid analysis, N-terminal amino acid sequence | Pseudomonas sp. |
86000 | - |
gel filtration | Pseudomonas sp. |
86000 | 95000 | gel filtration, sedimentation equilibrium centrifugation | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
AK1 | - |
Pseudomonas sp. AK1 | - |
AK1 | - |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
21.6 | - |
- |
Pseudomonas sp. |
Storage Stability | Organism |
---|---|
-20°C to 4°C, 50% loss of activity in a week | Pseudomonas sp. |
-60° to -170°C, stable for 6 months in potassium phosphate buffer, pH 6.5 | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-trimethylammoniobutanoate + 2-oxoglutarate + O2 | - |
Pseudomonas sp. | 3-hydroxy-4-trimethylammoniobutanoate + succinate + CO2 | - |
? | |
4-trimethylammoniobutanoate + 2-oxoglutarate + O2 | - |
Pseudomonas sp. AK1 | 3-hydroxy-4-trimethylammoniobutanoate + succinate + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 39000 + 1 * 37000, SDS-PAGE, amino acid analysis, N-terminal amino acid sequence | Pseudomonas sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Pseudomonas sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
34 | - |
gamma-butyrobetaine | - |
Pseudomonas sp. |